Ana Ardá Freire

Ikerbasque Research Associate, CIC bioGUNE

Currently works at the Chemical Glycobiology group at CIC bioGUNE (Bilbao).

Research line:

Chemical glycobiology.

Ramón y Cajal Fellow (2015-2020) and Juan de la Cierva Fellow (2009-2012).

Previous research experience at Bijvoet Center for Biomolecular Research (Utrecht, The Netherlands) and Centro de Investigaciones Biológicas (CIB-CSIC) (Madrid).

Doctor in Chemistry (2008) from Universidad de A Coruña.

Publications
Congresses & Proceedings

2024

Article / Probing altered receptor specificities of antigenically drifting human H3N2 viruses by chemoenzymatic synthesis, NMR, and modeling

Nature Communications

Unione, L.; Ammerlaan, A.N.A.; Bosman, G.P.; Uslu, E.; Liang, R.; Broszeit, F.; van der Woude, R.; Liu, Y.; Ma, S.; Liu, L.; Gómez-Redondo, M.; Bermejo, I.A.; Valverde, P.; Diercks, T.; Ardá, A.; de Vries, R.P.; Boons, G.J.

Article / The impact of glycosylation on the structure, function, and interactions of CD14

Glycobiology

Quintana, J.I.; Delgado, S.; Rábano, M.; Azkargorta, M.; Florencio-Zabaleta, M.; Unione, L.; dM Vivanco, M.; Elortza, F.; Jiménez-Barbero, J.; Ardá, A.

Article / Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue

Journal of Visualized Experiments

Bertuzzi, S.; Poveda, A.; Ardá, A.; Gimeno, A.; Jiménez-Barbero, J.

Article / Different roles of the heterodimer architecture of galectin-4 in selective recognition of oligosaccharides and lipopolysaccharides having ABH antigens

Journal of Biological Chemistry

Quintana, J.I.; Massaro, M.; Cagnoni, A.J.; Nuñez-Franco, R.; Delgado, S.; Jiménez-Osés, G.; Mariño, K.V.; Rabinovich, G.A.; Jiménez-Barbero, J.; Ardá, A.

Article / Exploring galectin interactions with human milk oligosaccharides and blood group antigens identifies BGA6 as a functional galectin-4 ligand

Journal of Biological Chemistry

Cagnoni, A.J.; Massaro, M.; Cutine, A.M.; Gimeno, A.; Pérez-Sáez, J.M.; Manselle Cocco, M.N.; Maller, S.M.; Di Lella, S.; Jiménez-Barbero, J.; Ardá, A.; Rabinovich, G.A.; Mariño, K.V.

Article / Insights into the recognition of hypermucoviscous Klebsiella pneumoniae clinical isolates by innate immune lectins of the Siglec and galectin families

Frontiers in Immunology

Campanero-Rhodes, M.A.; Martí, S.; Hernández-Ortiz, N.; Cubero, M.; Ereño-Orbea, J.; Ardá, A.; Jiménez-Barbero, J.; Ardanuy, C.; Solís, D.

Article / Unprecedented selectivity for homologous lectin targets: differential targeting of the viral receptors L-SIGN and DC-SIGN

Chemical Science

Delaunay, C.; Pollastri, S.; Thepaut, M.; Cavazzoli, G.; Belvisi, L.; Bouchikri, C.; Labiod, N.; Lasala, F.; Gimeno, A.; Franconetti, A.; Jimenez-Barbero, J.; Arda, A.; Delgado, R.; Bernardi, A.; Fieschi, F.

Article / Glycosylium Ions in Superacid Mimic the Transition State of Enzyme Reactions

Journal of the American Chemical Society

Armand, M.; Nin-Hill, A.; Ardá, A.; Berrino, E.; Désiré, J.; Martin-Mingot, A.; Michelet, B.; Jiménez-Barbero, J.; Blériot, Y.; Rovira, C.; Thibaudeau, S.

Review / Influence of Selective Deoxyfluorination on the Molecular Structure of Type-2 N-Acetyllactosamine

Journal of Organic Chemistry

Kurfirt, M.; St’astna, L.C.; Dracinsky, M.; Pohl, R.; Cisarova, I.; Jan, S.; Baka, M.; Hamala, V.; Canada, F.J.; Arda, A.; Jimenez-Barbero, J.; Karban, J.

Review / NMR investigations of glycan conformation, dynamics, and interactions

Progress in Nuclear Magnetic Resonance Spectroscopy

Angulo, J.; Ardá, A.; Bertuzzi, S.; Canales, A.; Ereño-Orbea, J.; Gimeno, A.; Gomez-Redondo, M.; Muñoz-García, J.C.; Oquist, P.; Monaco, S.; Poveda, A.; Unione, L.; Jiménez-Barbero, J.

2023

Article / Molecular Recognition of Glycan-Bearing Glycomacromolecules Presented at Membrane Surfaces by Lectins: An NMR View

ACS Omega

Lete, M.G.; Hoffmann, M.; Schomann, N.; Martínez-Castillo, A.; Peccati, F.; Konietzny, P.B.; Delgado, S.; Snyder, N.L.; Jiménez-Oses, G.; Abrescia, N.G.A.; Ardá, A.; Hartmann, L.; Jiménez-Barbero, J.

Article / Identification of a new DC-SIGN binding pentamannoside epitope within the complex structure of Candida albicans mannan

The Cell Surface

Krylov, V.B.; Gómez-Redondo, M.; Solovev, A.S.; Yashunsky, D.V.; Brown, A.J.P.; Stappers, M.H.T.; Gow, N.A.R.; Ardá, A.; Jiménez-Barbero, J.; Nifantiev, N.E.

Article / Biomimetic Tweezers for N-Glycans: Selective Recognition of the Core GlcNAc2 Disaccharide of the Sialylglycopeptide SGP

Chemistry - A European Journal

Milanesi, F.; Unione, L.; Ardá, A.; Nativi, C.; Jiménez-Barbero, J.; Roelens, S.; Francesconi, O.

Article / NMR Investigation of Protein-Carbohydrate Interactions: The Recognition of Glycans by Galectins Engineered with Fluorotryptophan Residues

Chemistry - A European Journal

Lete, M.G.; Franconetti, A.; Bertuzzi, S.; Delgado, S.; Azkargorta, M.; Elortza, F.; Millet, O.; Jiménez-Osés, G.; Arda, A.; Jiménez-Barbero, J.

Article / Targeting the Spike: Repurposing Mithramycin and Dihydroergotamine to Block SARS-CoV-2 Infection

ACS Omega

Stagnoli, S.; Macari, G.; Corsi, P.; Capone, B.; Vidaurrazaga, A.; Ereño-Orbea, J.; Ardá, A.; Polticelli, F.; Jiménez-Barbero, J.; Abrescia, N.G.; Coluzza, I.

Review / Exploring multivalent carbohydrate-protein interactions by NMR

Chemical Society Reviews

Quintana, J.I.; Atxabal, U.; Unione, L.; Ardá, A.; Jiménez-Barbero, J.

2022

Article / Assessing the Mobility of Severe Acute Respiratory Syndrome Coronavirus-2 Spike Protein Glycans by Structural and Computational Methods

Frontiers in Microbiology

Stagnoli, S.; Peccati, F.; Connell, S.R.; Martinez-Castillo, A.; Charro, D.; Millet, O.; Bruzzone, C.; Palazon, A.; Ardá, A.; Jiménez-Barbero, J.; Ereño-Orbea, J.; Abrescia, N.G.A.; Jiménez-Osés, G.

Article / Immobilization of Biantennary N-Glycans Leads to Branch Specific Epitope Recognition by LSECtin

ACS Central Science

Bertuzzi, S.; Peccati, F.; Serna, S.; Artschwager, R.; Notova, S.; Thépaut, M.; Jiménez-Osés, G.; Fieschi, F.; Reichardt, N.C.; Jiménez-Barbero, J.; Ardá, A.

Article / The SARS-CoV-2 Spike Glycoprotein Directly Binds Exogeneous Sialic Acids: A NMR View

Angewandte Chemie - International Edition

Unione, L.; Moure, M.J.; Lenza, M.P.; Oyenarte, I.; Ereño-Orbea, J.; Ardá, A.; Jiménez-Barbero, J.

Article / Oligosaccharide Presentation Modulates the Molecular Recognition of Glycolipids by Galectins on Membrane Surfaces

Pharmaceuticals

Lete, M.G.; Franconetti, A.; Delgado, S.; Jiménez-Barbero, J.; Ardá, A.

Article / Proceedings of workshop: “Neuroglycoproteins in health and disease”, INNOGLY cost action

Glycoconjugate Journal

Llop, E.; Ardá, A.; Zacco, E.; O’Flaherty, R.; García-Ayllón, M.S.; Aureli, M.; Frenkel-Pinter, M.; Reis, C.A.; Greiner-Tollersrud, O.K.; Cuchillo-Ibáñez, I.

2021

Article / Chemoenzymatic Synthesis of Complex N-Glycans of the Parasite S. mansoni to Examine the Importance of Epitope Presentation on DC-SIGN recognition

Angewandte Chemie - International Edition

Srivastava, A.D.; Unione, L.; Bunyatov, M.; Gagarinov, I.A.; Delgado, S.; Abrescia, N.G.A.; Ardá, A.; Boons, G.J.

Article / Minimal epitope for Mannitou IgM on paucimannose-carrying glycoproteins

Glycobiology

Robakiewicz, S.; Bridot, C.; Serna, S.; Gimeno, A.; Echeverria, B.; Delgado, S.; De Ruyck, J.; Semwal, S.; Charro, D.; Dansercoer, A.; Verstraete, K.; Azkargorta, M.; Van Noort, K.; Wilbers, R.H.P.; Savvides, S.N.; Abrescia, N.G.A.; Arda, A.; Reichardt, N.C.; Jiménez-Barbero, J.; Bouckaert, J.

Article / Cross‐linking effects dictate the preference of galectins to bind lacnac‐decorated hpma copolymers

International Journal of Molecular Sciences

Bertuzzi, S.; Gimeno, A.; Martinez‐castillo, A.; Lete, M.G.; Delgado, S.; Airoldi, C.; Tavares, M.R.; Bláhová, M.; Chytil, P.; Křen, V.; Abrescia, N.G.A.; Ardá, A.; Bojarová, P.; Jiménez‐barbero, J.

Article / Kinetic Studies of Acetyl Group Migration between the Saccharide Units in an Oligomannoside Trisaccharide Model Compound and a Native Galactoglucomannan Polysaccharide

ChemBioChem

Lassfolk, R.; Bertuzzi, S.; Ardá, A.; Wärnå, J.; Jiménez-Barbero, J.; Leino, R.

Article / Selective <sup>13</sup>C-Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins

Angewandte Chemie - International Edition

Moure, M.J.; Gimeno, A.; Delgado, S.; Diercks, T.; Boons, G.J.; Jiménez-Barbero, J.; Ardá, A.

Article / The two domains of human galectin-8 bind sialyl-and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR

RSC Chemical Biology

Gómez-Redondo, M.; Delgado, S.; Núñez-Franco, R.; Jiménez-Osés, G.; Ardá, A.; Jiménez-Barbero, J.; Gimeno, A.

Article / Glycosyl Oxocarbenium Ions: Structure, Conformation, Reactivity, and Interactions

Accounts of Chemical Research

Franconetti, A.; Ardá, A.; Asensio, J.L.; Blériot, Y.; Thibaudeau, S.; Jiménez-Barbero, J.

Article / Galectin-4 N-Terminal Domain: Binding Preferences Toward A and B Antigens With Different Peripheral Core Presentations

Frontiers in Chemistry

Quintana, J.I.; Delgado, S.; Núñez-Franco, R.; Cañada, F.J.; Jiménez-Osés, G.; Jiménez-Barbero, J.; Ardá, A.

Article / Exploration of galectin ligands displayed on gram-negative respiratory bacterial pathogens with different cell surface architectures

Biomolecules

Campanero-Rhodes, M.A.; Kalograiaki, I.; Euba, B.; Llobet, E.; Ardá, A.; Jiménez-Barbero, J.; Garmendia, J.; Solís, D.

Article / Synthesis and chelation study of a fluoroionophore and a glycopeptide based on an aza crown iminosugar structure

Carbohydrate Research

Bordes, A.; Poveda, A.; Fontelle, N.; Ardá, A.; Guillard, J.; Ruan, Y.B.; Marrot, J.; Imaeda, S.; Kato, A.; Désiré, J.; Xie, J.; Jiménez-Barbero, J.; Blériot, Y.

Article / Insight into the Ferrier Rearrangement by Combining Flash Chemistry and Superacids

Angewandte Chemie - International Edition

Bhuma, N.; Lebedel, L.; Yamashita, H.; Shimizu, Y.; Abada, Z.; Ardá, A.; Désiré, J.; Michelet, B.; Martin-Mingot, A.; Abou-Hassan, A.; Takumi, M.; Marrot, J.; Jiménez-Barbero, J.; Nagaki, A.; Blériot, Y.; Thibaudeau, S.

Article / Recent advances in the application of NMR methodologies to analyze the conformation, dynamics, and interactions of saccharides

Carbohydrate Chemistry

Arda, A.; Atxabal, U.; Bertuzzi, S.; Canales, A.; Cañada, F.J.; Gimeno, A.; Ereño-Orbea, J.; De Toro, B.F.; Franconetti, A.; Gómez-Redondo, M.; Lete, M.G.; Lenza, M.P.; Martínez, J.D.; Moure, M.J.; Poveda, A.; Quintana, J.I.; Valverde, P.; Jiménez-Barbero, J.

Review / NMR of glycoproteins: profiling, structure, conformation and interactions

Current Opinion in Structural Biology

Unione, L.; Ardá, A.; Jiménez-Barbero, J.; Millet, O.

2023

Invited talk | GLYCAN-LECTIN MOLECULAR RECOGNITION:NEW NMR STARTEGIES AND KEY MOLECULAR MECHANISMS

XIII Jornadas de Carbohidratos, GE HiC, RSEQ - Invited talk

Ana Arda

Invited talk | GLYCAN-LECTIN MOLECULAR RECOGNITION:NEW NMR STRATEGIES AND KEY MOLECULAR MECHANISMS

Ana Arda

Invited talk | Structure guided glycoconjugate vaccine design

Eurocarb 21- European Carbohydrate Symposium - Invited talk

Ana Arda and Roberto Adamo

Invited talk | NMR applied to glycoproteins and molecular recognition

Jornada de otoño del GERMN - Invited talk

Ana Arda

Invited talk | NMR applied to glycoproteins and glycan mediated molecular recognition events

RCS Carbohydrate Winter Meeting - Invited talk

Ana Arda

Invited talk | Glycan-lectin molecular recognition: new NMR strategies and key molecular mechanisms

Seminar at the Biomolecular Systems Department, Max Planck Institute of Colloids and Interfaces - Invited talk

Ana Arda

Poster presentation | Molecular insights into Galectin-4 binding to oligo- and lipopolysaccharides containing the ABH histo-blood antigens

2023 Carbohydrates Gordon Research Conference - Poster presentation

Jon I. Quintana, Sandra Delgado, Jesús Jiménez-Barbero, Ana Ardá

2021

Poster presentation | Targeting SARS-CoV-2 spike protein glycans by structural and computational methods

Congress Microscopy at the Frontiers of Science - Poster presentation

Soledad Stagnoli*, Francesca Peccati, Sean R Connell, Ane Martinez-Castillo, Diego Charro, Oscar Millet, Chiara Bruzzone, Asis Palazon, Ana Ardá, Jesús Jiménez-Barbero, June Ereño-Orbea, Nicola GA Abrescia, Gonzalo Jiménez-Osés