Rafael Pulido

Ikerbasque Research Professor, Biobizkaia Health Research Institute

Group leader of the Cancer Biomarkers group at Biobizkaia Health Research Institute.

Research line:

Role of protein phosphatases and kinases in human cancer.

Previous research experience in CSIC (Spain), The Salk Institute (United States), Dana-Farber Cancer Institute (United States), Max Planck Institut fur Biochemie (Germany) and Centro de Investigación Príncipe Felipe (Spain).

Doctor in Biology from Universidad Autónoma de Madrid, Spain, 1990.

Publicaciones

2024

Article / The lincRNA JUNI regulates the stress-dependent induction of c-Jun, cellular migration and survival through the modulation of the DUSP14-JNK axis

Oncogene

Kumar, V.; Sabaté-Cadenas, X.; Soni, I.; Stern, E.; Vias, C.; Ginsberg, D.; Romá-Mateo, C.; Pulido, R.; Dodel, M.; Mardakheh, F.K.; Shkumatava, A.; Shaulian, E.

Article / Predicting Survival of Metastatic Clear Cell Renal Cell Cancer Treated with VEGFR-TKI-Based Sequential Therapy

Cancers

Angulo, J.C.; Larrinaga, G.; Lecumberri, D.; Iturregui, A.M.; Solano-Iturri, J.D.; Lawrie, C.H.; Armesto, M.; Dorado, J.F.; Nunes-Xavier, C.E.; Pulido, R.; Manini, C.; López, J.I.

Article / Potentiation by Protein Synthesis Inducers of Translational Readthrough of Pathogenic Premature Termination Codons in PTEN Isoforms

Cancers

Torices, L.; Nunes-Xavier, C.E.; Pulido, R.

Article / A functional role for glycosylated B7-H5/VISTA immune checkpoint protein in metastatic clear cell renal cell carcinoma

iScience

Emaldi, M.; Alamillo-Maeso, P.; Rey-Iborra, E.; Mosteiro, L.; Lecumberri, D.; Pulido, R.; López, J.I.; Nunes-Xavier, C.E.

Article / Impact of B7-H3 expression on metastasis, immune exhaustion and JAK/STAT and PI3K/AKT pathways in clear cell renal cell carcinoma

OncoImmunology

Emaldi, M.; Rey-Iborra, E.; Marín, Á.; Mosteiro, L.; Lecumberri, D.; Øyjord, T.; Roncier, N.; Mælandsmo, G.M.; Angulo, J.C.; Errarte, P.; Larrinaga, G.; Pulido, R.; López, J.I.; Nunes-Xavier, C.E.

Short Survey / B7-H3: a robust target for immunotherapy in prostate cancer

Trends in Cancer

Pulido, R.; López, J.I.; Nunes-Xavier, C.E.

2023

Article / The expression pattern of pyruvate dehydrogenase kinases predicts prognosis and correlates with immune exhaustion in clear cell renal cell carcinoma

Scientific Reports

Nunes-Xavier, C.E.; Emaldi, M.; Mingo, J.; Øyjord, T.; Mælandsmo, G.M.; Fodstad, Ø.; Errarte, P.; Larrinaga, G.; Llarena, R.; López, J.I.; Pulido, R.

Article / Novel anti-PTEN C2 domain monoclonal antibodies to analyse the expression and function of PTEN isoform variants

PLoS ONE

Torices, L.; Nunes-Xavier, C.E.; López, J.I.; Pulido, R.

Article / Functional analysis of PTEN variants of unknown significance from PHTS patients unveils complex patterns of PTEN biological activity in disease

European Journal of Human Genetics

Torices, L.; Mingo, J.; Rodríguez-Escudero, I.; Fernández-Acero, T.; Luna, S.; Nunes-Xavier, C.E.; López, J.I.; Mercadillo, F.; Currás, M.; Urioste, M.; Molina, M.; Cid, V.J.; Pulido, R.

Article / Hopes on immunotherapy targeting B7-H3 in neuroblastoma

Translational Oncology

Pulido, R.; Nunes-Xavier, C.E.

Article / Correlation of expression of Major Vault Protein with androgen receptor and immune checkpoint protein B7-H3, and with poor prognosis in prostate cancer

Pathology Research and Practice

Nunes-Xavier, C.E.; Emaldi, M.; Guldvik, I.J.; Ramberg, H.; Taskén, K.A.; Mælandsmo, G.M.; Fodstad, Ø.; Llarena, R.; Pulido, R.; López, J.I.

Editorial / Editorial: Cell and Developmental Signalling in Neuroblastoma

Frontiers in Cell and Developmental Biology

Pulido, R.; Nunes-Xavier, C.E.

Review / Hereditable variants of classical protein tyrosine phosphatase genes: Will they prove innocent or guilty?

Frontiers in Cell and Developmental Biology

Hendriks, W.J.A.J.; van Cruchten, R.T.P.; Pulido, R.

2022

Article / Heterogeneous Expression and Subcellular Localization of Pyruvate Dehydrogenase Complex in Prostate Cancer

Frontiers in Oncology

Nunes-Xavier, C.E.; Mingo, J.; Emaldi, M.; Flem-Karlsen, K.; Mælandsmo, G.M.; Fodstad, Ø.; Llarena, R.; López, J.I.; Pulido, R.

2021

Article / MMADHC premature termination codons in the pathogenesis of cobalamin D disorder: Potential of translational readthrough reconstitution

Molecular Genetics and Metabolism Reports

Torices, L.; de las Heras, J.; Arango-Lasprilla, J.C.; Cortés, J.M.; Nunes-Xavier, C.E.; Pulido, R.

Article / A global analysis of the reconstitution of PTEN function by translational readthrough of PTEN pathogenic premature termination codons

Human Mutation

Luna, S.; Torices, L.; Mingo, J.; Amo, L.; Rodríguez-Escudero, I.; Ruiz-Ibarlucea, P.; Erramuzpe, A.; Cortés, J.M.; Tejada, M.I.; Molina, M.; Nunes-Xavier, C.E.; López, J.I.; Cid, V.J.; Pulido, R.

Article / (Pro)renin receptor is a novel independent prognostic marker in invasive urothelial carcinoma of the bladder

Cancers

Larrinaga, G.; Calvete-Candenas, J.; Solano-Iturri, J.D.; Martín, A.M.; Pueyo, A.; Nunes-Xavier, C.E.; Pulido, R.; Dorado, J.F.; López, J.I.; Angulo, J.C.

Article / Immune checkpoint B7-H3 protein expression is associated with poor outcome and androgen receptor status in prostate cancer

Prostate

Nunes-Xavier, C.E.; Kildal, W.; Kleppe, A.; Danielsen, H.E.; Wæhre, H.; Llarena, R.; Mælandsmo, G.M.; Fodstad, Ø.; Pulido, R.; López, J.I.

Review / Protein Tyrosine Phosphatases in Neuroblastoma: Emerging Roles as Biomarkers and Therapeutic Targets

Frontiers in Cell and Developmental Biology

Nunes-Xavier, C.E.; Zaldumbide, L.; Mosteiro, L.; López-Almaraz, R.; García de Andoin, N.; Aguirre, P.; Emaldi, M.; Torices, L.; López, J.I.; Pulido, R.

2020

Article / Oligometastatic prostate adenocarcinoma. Clinical-pathologic study of a histologically under-recognized prostate cancer

Journal of Personalized Medicine

Manini, C.; González, A.; Büchser, D.; García-Olaverri, J.; Urresola, A.; Ezquerro, A.; Fernández, I.; Llarena, R.; Zabalza, I.; Pulido, R.; Carracedo, A.; Gómez-Iturriaga, A.; López, J.I.

Article / Brain circuit alterations and cognitive disability in late-onset cobalamin d disorder

Journal of Clinical Medicine

Heras, J.D.L.; Diez, I.; Jimenez-Marin, A.; Cabrera, A.; Ramos-Usuga, D.; Diaz-Fernandez, M.V.; Torices, L.; Nunes-Xavier, C.E.; Pulido, R.; Arango-Lasprilla, J.C.; Cortes, J.M.

Review / Modeling human disease in yeast: recreating the PI3K-PTEN-Akt signaling pathway in Saccharomyces cerevisiae

International Microbiology

Coronas-Serna, J.M.; Valenti, M.; del Val, E.; Fernández-Acero, T.; Rodríguez-Escudero, I.; Mingo, J.; Luna, S.; Torices, L.; Pulido, R.; Molina, M.; Cid, V.J.

2019

Article / Protein tyrosine phosphatase PTPN1 modulates cell growth and associates with poor outcome in human neuroblastoma

Diagnostic Pathology

Nunes-Xavier, C.E.; Aurtenetxe, O.; Zaldumbide, L.; López-Almaraz, R.; Erramuzpe, A.; Cortés, J.M.; López, J.I.; Pulido, R.

Article / Expression of human PTEN-L in a yeast heterologous model unveils specific N-terminal motifs controlling PTEN-L subcellular localization and function

Cells

Fernández-Acero, T.; Bertalmio, E.; Luna, S.; Mingo, J.; Bravo-Plaza, I.; Rodríguez-Escudero, I.; Molina, M.; Pulido, R.; Cid, V.J.

Article / Precise definition of PTEN C-terminal epitopes and its implications in clinical oncology

npj Precision Oncology

Mingo, J.; Luna, S.; Gaafar, A.; Nunes-Xavier, C.E.; Torices, L.; Mosteiro, L.; Ruiz, R.; Guerra, I.; Llarena, R.; Angulo, J.C.; López, J.I.; Pulido, R.

Article / Precise immunodetection of PTEN protein in human neoplasia

Cold Spring Harbor Perspectives in Medicine

Pulido, R.; Mingo, J.; Gaafar, A.; Nunes-Xavier, C.E.; Luna, S.; Torices, L.; Angulo, J.C.; López, J.I.

Article / The coexpression of fibroblast activation protein (FAP) and basal-type markers (CK 5/6 and CD44) predicts prognosis in high-grade invasive urothelial carcinoma of the bladder

Human Pathology

Calvete, J.; Larrinaga, G.; Errarte, P.; Martín, A.M.; Dotor, A.; Esquinas, C.; Nunes-Xavier, C.E.; Pulido, R.; López, J.I.; Angulo, J.C.

Editorial / Dual specificity phosphatases: From molecular mechanisms to biological function

International Journal of Molecular Sciences

Pulido, R.; Lang, R.

Review / Dual-specificity phosphatases in neuroblastoma cell growth and differentiation

International Journal of Molecular Sciences

Nunes-Xavier, C.E.; Zaldumbide, L.; Aurtenetxe, O.; López-Almaraz, R.; López, J.I.; Pulido, R.

Review / A Critical Insight into the Clinical Translation of PD-1/PD-L1 Blockade Therapy in Clear Cell Renal Cell Carcinoma

Current Urology Reports

Nunes-Xavier, C.E.; Angulo, J.C.; Pulido, R.; López, J.I.

Review / The role of protein tyrosine phosphatases in prostate cancer biology

Biochimica et Biophysica Acta - Molecular Cell Research

Nunes-Xavier, C.E.; Mingo, J.; López, J.I.; Pulido, R.

2018

Article / DUSP5 associates with poor prognosis in human neuroblastoma

Exp. Mol. Pathol.

Aurtenetxe, O., Zaldumbide, L., Erramuzpe, A., López, R., López, J.I, Cortés, J.M., Pulido, R., Nunes-Xavier. C.E.

Article / Potential impact of PD-L1 (SP-142) immunohistochemical heterogeneity in clear cell renal cell carcinoma immunotherapy

Pathology Research and Practice

López, J.I.; Pulido, R.; Cortés, J.M.; Angulo, J.C.; Lawrie, C.H.

Article / Loss of PD-L1 (SP-142) expression characterizes renal vein tumor thrombus microenvironment in clear cell renal cell carcinoma

Annals of Diagnostic Pathology

López, J.I.; Pulido, R.; Lawrie, C.H.; Angulo, J.C.

Article / High levels of intratumor heterogeneity characterize the expression of epithelial-mesenchymal transition markers in high-grade clear cell renal cell carcinoma

Annals of Diagnostic Pathology

Guarch, R.; Lawrie, C.H.; Larrinaga, G.; Angulo, J.C.; Pulido, R.; López, J.I.

Article / DUSP5 expression associates with poor prognosis in human neuroblastoma

Experimental and Molecular Pathology

Aurtenetxe, O.; Zaldumbide, L.; Erramuzpe, A.; López, R.; López, J.I.; Cortés, J.M.; Pulido, R.; Nunes-Xavier, C.E.

Article / A pathogenic role for germline PTEN variants which accumulate into the nucleus

European Journal of Human Genetics

Mingo, J.; Rodríguez-Escudero, I.; Luna, S.; Fernández-Acero, T.; Amo, L.; Jonasson, A.R.; Zori, R.T.; López, J.I.; Molina, M.; Cid, V.J.; Pulido, R.

Review / Proteinaceous regulators and inhibitors of protein tyrosine phosphatases

Molecules

Hendriks, W.; Bourgonje, A.; Leenders, W.; Pulido, R.

Review / PTEN inhibition in human disease therapy

Molecules

Pulido, R.

2017

Article / CD34 immunostaining enhances a distinct pattern of intratumor angiogenesis with prognostic implications in clear cell renal cell carcinoma

APMIS

López, J.I.; Erramuzpe, A.; Guarch, R.; Cortés, J.M.; Pulido, R.; Llarena, R.; Angulo, J.C.

Article / Insights into the pathological mechanisms of p85α mutations using a yeast-based phosphatidylinositol 3-kinase model

Bioscience Reports

Oliver, M.D.; Fernández-Acero, T.; Luna, S.; Rodríguez-Escudero, I.; Molina, M.; Pulido, R.; Cid, V.J.

2016

Chapter / Protein Tyrosine Phosphatases: Methods and Protocols

Pulido, R.

Alonso, A., Nunes-Xavier, C., Bayón, Y., Pulido, R.

Chapter / PTEN: Methods and Protocols

Salmena, L., Stambolic, V.

Gil, A., López, J.I., Pulido, R.

Chapter / Protein Tyrosine Phosphatases: Methods and Protocols

Pulido, R.

Nunes-Xavier, C., Pulido, R.

Chapter / Protein Tyrosine Phosphatases: Methods and Protocols

Pulido, R.

Luna, S., Mingo, J., Aurtenetxe, O., Blanco, L., Amo, L., Schepens, J., Hendriks, W.J., Pulido, R.

Article / Low-grade metastases in high-grade clear cell renal cell carcinomas. A clinico-pathological study of 4 cases with an insight into the role of mesenchymal-to-epithelial transition process

Ann. Diagn. Pathol.

López, J.I., Mosteiro, L., Guarch, R., Larrinaga, G., Pulido, R., Angulo, J.C.

Article / One-tube-only standardized Site-directed mutagenesis: An alternative approach to generate amino acid substitution collections

PLoS ONE

Mingo, J.; Erramuzpe, A.; Luna, S.; Aurtenetxe, O.; Amo, L.; Diez, I.; Schepens, J.T.G.; Hendriks, W.J.A.J.; Cortés, J.M.; Pulido, R.

Article / Fibroblast activation protein predicts prognosis in clear cell renal cell carcinoma

Human Pathology

López, J.I.; Errarte, P.; Erramuzpe, A.; Guarch, R.; Cortés, J.M.; Angulo, J.C.; Pulido, R.; Irazusta, J.; Llarena, R.; Larrinaga, G.

Article / The expression of fibroblast activation protein in clear cell renal cell carcinomas is associated with synchronous lymph node metastases

PLoS ONE

Errarte, P.; Guarch, R.; Pulido, R.; Blanco, L.; Nunes-Xavier, C.E.; Beitia, M.; Gil, J.; Angulo, J.C.; Lopez, J.I.; Larrinaga, G.

Article / The extended human PTPome: A growing tyrosine phosphatase family

FEBS Journal

Alonso, A.; Pulido, R.

Article / PTEN recruitment controls synaptic and cognitive function in Alzheimer's models

Nature Neuroscience

Knafo, S.; Sánchez-Puelles, C.; Palomer, E.; Delgado, I.; Draffin, J.E.; Mingo, J.; Wahle, T.; Kaleka, K.; Mou, L.; Pereda-Perez, I.; Klosi, E.; Faber, E.B.; Chapman, H.M.; Lozano-Montes, L.; Ortega-Molina, A.; Ordóñez-Gutiérrez, L.; Wandosell, F.; Viña, J.; Dotti, C.G.; Hall, R.A.; Pulido, R.; Gerges, N.Z.; Chan, A.M.; Spaller, M.R.; Serrano, M.; Venero, C.; Esteban, J.A.

Article / Low-grade metastases in high-grade clear cell renal cell carcinomas A clinicopathologic study of 4 cases with an insight into the role of mesenchymal-to-epithelial transition process

Annals of Diagnostic Pathology

López, J.I.; Mosteiro, L.; Guarch, R.; Larrinaga, G.; Pulido, R.; Angulo, J.C.

Article / Snail heterogeneity in clear cell renal cell carcinoma

BMC Cancer

Zaldumbide, L.; Erramuzpe, A.; Guarch, R.; Pulido, R.; Cort�s, J.M.; L�pez, J.I.

2015

Article / PTEN: a yin-yang master regulator protein in human health and disease

Methods

Pulido, R

Article / Yeast-based methods to assess PTEN phosphoinositide phosphatase activity in vivo

Methods

Rodríguez-Escudero, I.; Fernández-Acero, T.; Bravo, I.; Leslie, N.R.; Pulido, R.; Molina, M.; Cid, V.J.

Article / PTEN: A yin-yang master regulator protein in health and disease

Methods

Pulido, R.

Article / A functional dissection of PTEN N-terminus: Implications in PTEN subcellular targeting and tumor suppressor activity

PLoS ONE

Gil, A.; Rodríguez-Escudero, I.; Stumpf, M.; Molina, M.; Cid, V.J.; Pulido, R.

Editorial / A rapid guide to PTEN function

Methods

Pulido, R.

2014

Article / PTPRR (protein tyrosine phosphatase, receptor type, R)

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Erkens, M., Kremer, H., Pulido, R., Hendriks, W.

Review / A unified nomenclature and amino acid numbering for human PTEN

Science Signaling

Pulido, R.; Baker, S.J.; Barata, J.T.; Carracedo, A.; Cid, V.J.; Chin-Sang, I.D.; Davé, V.; Den Hertog, J.; Devreotes, P.; Eickholt, B.J.; Eng, C.; Furnari, F.B.; Georgescu, M.M.; Gericke, A.; Hopkins, B.; Jiang, X.; Lee, S.R.; Lösche, M.; Malaney, P.; Matias-Guiu, X.; Molina, M.; Pandolfi, P.P.; Parsons, R.; Pinton, P.; Rivas, C.; Rocha, R.M.; Rodríguez, M.S.; Ross, A.H.; Serrano, M.; Stambolic, V.; Stiles, B.; Suzuki, A.; Tan, S.S.; Tonks, N.K.; Trotman, L.C.; Wolff, N.; Woscholski, R.; Wu, H.; Leslie, N.R.

Review / Dual-specificity phosphatases as molecular targets for inhibition in human disease

Antioxidants and Redox Signaling

Ríos, P.; Nunes-Xavier, C.E.; Tabernero, L.; Köhn, M.; Pulido, R.

2013

Article / PTPsemerge as PIPs: Protein tyrosine phosphatases with lipid-phosphatase activities in human disease

Human Molecular Genetics

Pulido, R.; Stoker, A.W.; Hendriks, W.J.A.J.

Article / PINK1 regulates histone H3 trimethylation and gene expression by interaction with the polycomb protein EED/WAIT1

Proceedings of the National Academy of Sciences of the United States of America

Berthier, A.; Jiménez-Sáinz, J.; Pulido, R.

Review / Protein tyrosine phosphatases as novel targets in breast cancer therapy

Biochimica et Biophysica Acta - Reviews on Cancer

Nunes-Xavier, C.E.; Martín-Pérez, J.; Elson, A.; Pulido, R.

Review / Protein tyrosine phosphatase variants in human hereditary disorders and disease susceptibilities

Biochimica et Biophysica Acta - Molecular Basis of Disease

Hendriks, W.J.A.J.; Pulido, R.