Paola Fucini

Ikerbasque Research Professor, PIE - UPV/EHU

Ikerbasque Research Professor at PIE - Research Centre for Experimental Marine Biology & Biotechnology.

Research line:

Ribosome Structural Biology.

Previous research experience at Cluster of Excellence for Macromolecular Complexes (CEF-MC), Buchmann Institute for Molecular Life Science (BMLS); Institut für Organische Chemie und Chemische Biologie, J. W. Goethe-Universität Frankfurt; and CIC bioGUNE.

Dr. rer. nat. (Ph.D.) conducted at the Max-Planck-Institut für Biochemie, Martinsried, Germany. Obtained from the Ludwig-Maximilians-Universität, München.

Argitalpenak
Kongresuak eta Proceedings

2021

Article / Orthoformimycin inhibits translation elongation by displacing the A-site tRNA and preventing peptide bond formation

bioRxiv

Schedlbauer A., Kaminishi T., Fabbretti A., Milón P., Han X., Ochoa-Lizarralde B., Capuni R., Gualerzi C.O., Connell S.R., Fucini P.

Article / A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit

Science Advances

Schedlbauer, A.; Iturrioz, I.; Ochoa-Lizarralde, B.; Diercks, T.; Lopez-Alonso, J.P.; Lavin, J.L.; Kaminishi, T.; Capuni, R.; Dhimole, N.; de Astigarraga, E.; Gil-Carton, D.; Fucini, P.; Connell, S.R.

Article / Histone mRNA is subject to 3′ uridylation and re-adenylation in Aspergillus nidulans

Molecular Microbiology

Mossanen-Parsi, A.; Parisi, D.; Browne-Marke, N.; Bharudin, I.; Connell, S.R.; Mayans, O.; Fucini, P.; Morozov, I.Y.; Caddick, M.X.

2020

Article / Visualizing 30S decoding site folding facilitated by ribosome assembly factors

bioRxiv

Schedlbauer A., Kaminishi, T., López-Alonso JP., Iturrioz I., Ochoa-Lizarralde B., Gil-Carton D., Fucini P., Connell SR.

Article / Structural insights into Pseudomonas aeruginosa Type six secretion system exported effector 8

Journal of Structural Biology

González-Magaña, A.; Sainz-Polo, M.Á.; Pretre, G.; Çapuni, R.; Lucas, M.; Altuna, J.; Montánchez, I.; Fucini, P.; Albesa-Jové, D.

Article / Backbone and sidechain NMR assignments for the ribosome maturation factor RbfA from Escherichia coli

Biomolecular NMR Assignments

Schedlbauer, A.; Iturrioz, I.; Ochoa-Lizarralde, B.; Çapuni, R.; Han, X.; de Astigarraga, E.; Diercks, T.; Fucini, P.; Connell, S.R.

Article / Backbone and sidechain NMR assignments for the ribosome maturation factor RimP from Escherichia coli

Biomolecular NMR Assignments

Schedlbauer, A.; Ochoa-Lizarralde, B.; Iturrioz, I.; Çapuni, R.; Diercks, T.; de Astigarraga, E.; Fucini, P.; Connell, S.R.

2018

Article / The third-generation tetracycline, KBP-7072, exploits and reveals a new potential of the primary tetracycline binding pocket

bioRxiv

Tatsuya Kaminishi, Andreas Schedlbauer, Borja Ochoa-Lizarralde, Elisa de Astigarraga, Retina Çapuni, Fred Yang, Vincent Benn, Qingmei Liu, Xiaojuan Tan, Min Zhang, Sean R. Connell and Paola Fucini

2017

Article / RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket

Nucleic Acids Research

Pedro Lopez-Alonso, J.; Kaminishi, T.; Kikuchi, T.; Hirata, Y.; Iturrioz, I.; Dhimole, N.; Schedlbauer, A.; Hase, Y.; Goto, S.; Kurita, D.; Muto, A.; Zhou, S.; Naoe, C.; Mills, D.J.; Gil-Carton, D.; Takemoto, C.; Himeno, H.; Fucini, P.; Connell, S.R.

Article / Structure of a 30S pre-initiation complex stalled by GE81112 reveals structural parallels in bacterial and eukaryotic protein synthesis initiation pathways

Nucleic acids research

López-Alonso, J.P.; Fabbretti, A.; Kaminishi, T.; Iturrioz, I.; Brandi, L.; Gil-Carton, D.; Gualerzi, C.O.; Fucini, P.; Connell, S.R.

2016

Article / The novel aminomethylcycline omadacycline has high specificity for the primary tetracycline-binding site on the bacterial ribosome

Antibiotics

Heidrich, C.G.; Mitova, S.; Schedlbauer, A.; Connell, S.R.; Fucini, P.; Steenbergen, J.N.; Berens, C.

Article / Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding

Proceedings of the National Academy of Sciences of the United States of America

Fabbretti, A.; Schedlbauer, A.; Brandi, L.; Kaminishi, T.; Giuliodori, A.M.; Garofalo, R.; Ochoa-Lizarralde, B.; Takemoto, C.; Yokoyama, S.; Connell, S.R.; Gualerzi, C.O.; Fucini, P.

2015

Article / Crystallographic characterization of the ribosomal binding site and molecular mechanism of action of Hygromycin A

Nucleic Acids Research

Kaminishi, T.; Schedlbauer, A.; Fabbretti, A.; Brandi, L.; Ochoa-Lizarralde, B.; He, C.G.; Milón, P.; Connell, S.R.; Gualerzi, C.O.; Fucini, P.

Article / Structural characterization of an alternative mode of tigecycline binding to the bacterial ribosome

Antimicrobial Agents and Chemotherapy

Schedlbauer, A.; Kaminishi, T.; Ochoa-Lizarralde, B.; Dhimole, N.; Zhou, S.; López-Alonso, J.P.; Connell, S.R.; Fucini, P.

2013

Article / Solid-state NMR enhanced by dynamic nuclear polarization as a novel tool for ribosome structural biology

Journal of Biomolecular NMR

Gelis, I.; Vitzthum, V.; Dhimole, N.; Caporini, M.A.; Schedlbauer, A.; Carnevale, D.; Connell, S.R.; Fucini, P.; Bodenhausen, G.

2022

Oral presentation | Revealing the mechanism of action of universally conserved ribosome assembly factors that determine bacterial fitness and antimicrobial resistance (English title); Revelando el mecanismo de acción de los factores de ensamblaje de ribosoma universalmente

MikrobioGUNE, 2nd Basque Microbiology Meeting - Oral presentation

Xu Han , Borja Ochoa-Lizarralde , Sean R Connell and Paola Fucini

2020

Invited talk | Ribosomes in antibiotic action and resistance

Resistance to antibiotics. A One Health vision: Healthy people, healthy animals and a safe environment - Invited talk

Paola Fucini & Sean Connell

Invited talk | Establishing molecular tools to combat and survey microbial pathogens

Master Program in Environmental Contamination and Toxicology - Invited talk

Paola Fucini

2019

Poster presentation | A Cryo-EM and NMR approach unravels RbfA’s role in ribosome assembly

Ribosome Structure and Function Conference - Poster presentation

Schedlbauer A., Çapuni R., Iturrioz I., Ochoa-Lizarralde B., de Astigarraga E., Connell SR and Paola Fucini

2018

Oral presentation | Structural studies on novel translational inhibitors: toward the development of antibiotics with a new mechanism of action

MikrobioGUNE, 1st Basque Microbiology Meeting - Oral presentation

Paola Fucini

2016

Oral presentation | Inhibition of translation initiation complex formation by GE81112 unravels a 16S rRNA structural switch involved in P-site decoding

Ribosome Structure and Function - Oral presentation

Paola Fucini

2014

Invited talk | An NMR approach to study co-translational protein folding and sorting: Getting ready for THE NASCENT CODE

VII Biennial Meeting of the Nuclear Magnetic Resonance Group of the Spanish Royal Society of Chemistry / IV Iberian NMR Meeting / VI Ibero-American NMR Meeting - Invited talk

Paola Fucini